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human interleukin 4  (R&D Systems)


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    R&D Systems human interleukin 4
    Human Interleukin 4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1026 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+interleukin+4/Recombinant+Human+IL-4+Protein/pmc13093023-45-54-61
    Average 96 stars, based on 1026 article reviews
    human interleukin 4 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Differential Production of Midkine and Pleiotrophin by Innate APCs upon Stimulation through Nucleic Acid-Sensing TLRs
    Article Snippet: Macrophages were differentiated from monocytes cultured under adherence conditions for 6 days in RPMI media (Gibco, UK) supplemented with 50 ng/ml human recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D, UK). .. MDDCs were obtained from monocytes cultured under adherence conditions for 6 days in RPMI media supplemented with 50 ng/ml of human recombinant interleukin-4 (IL-4; R&D, UK) and 50 ng/ml GM-CSF. .. mDCs and pDCs were isolated from PBMCs by Myeloid Dendritic Cell Isolation kit, CD1c (BDCA-1) + Dendritic Cell Isolation kit, and Plasmacytoid Dendritic Cell Isolation kit II (Miltenyi Biotech, Germany) according to the manufacturer's instructions.

    Article Title: Functional characterization of biodegradable nanoparticles as antigen delivery system
    Article Snippet: .. Briefly, isolated PBMCs were allowed to adhere to plastic in 6-well plates for 2 h. Adherent monocytes were washed with RPMI 1640 medium and were then cultured for 6 days in DC culture medium supplemented with 50 ng of recombinant granulocyte-macrophage colony-stimulating factor (rGM-CSF; R&D Systems, Minneapolis, Minn.) per mL and 1000 U of recombinant interleukin-4 (rIL-4; R&D Systems, Minneapolis, Minn.) per mL. ..

    Recombinant:

    Article Title: Differential Production of Midkine and Pleiotrophin by Innate APCs upon Stimulation through Nucleic Acid-Sensing TLRs
    Article Snippet: Macrophages were differentiated from monocytes cultured under adherence conditions for 6 days in RPMI media (Gibco, UK) supplemented with 50 ng/ml human recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D, UK). .. MDDCs were obtained from monocytes cultured under adherence conditions for 6 days in RPMI media supplemented with 50 ng/ml of human recombinant interleukin-4 (IL-4; R&D, UK) and 50 ng/ml GM-CSF. .. mDCs and pDCs were isolated from PBMCs by Myeloid Dendritic Cell Isolation kit, CD1c (BDCA-1) + Dendritic Cell Isolation kit, and Plasmacytoid Dendritic Cell Isolation kit II (Miltenyi Biotech, Germany) according to the manufacturer's instructions.

    Article Title: A phase I dose-escalation clinical trial of a peptide-based human papillomavirus therapeutic vaccine with Candida skin test reagent as a novel vaccine adjuvant for treating women with biopsy-proven cervical intraepithelial neoplasia 2/3
    Article Snippet: .. Autologous dendritic cells were established by growing monocytes in the presence of granulocyte monocyte-colony stimulating factor (50 ng/mL, Sanofi-Aventis, #420039) and recombinant interleukin-4 (100 U/mL, R&D Systems, # 204-IL-050) for 7 d, and were matured by 48-h culture in wells containing irradiated mouse L-cells expressing CD40 ligands. .. CD3 T cells were magnetically selected (Pan T Cell Isolation Kit II, Miltenyi Biotec, #130-096-535) from CD14-depleted PBMCs.

    Article Title: Functional characterization of biodegradable nanoparticles as antigen delivery system
    Article Snippet: .. Briefly, isolated PBMCs were allowed to adhere to plastic in 6-well plates for 2 h. Adherent monocytes were washed with RPMI 1640 medium and were then cultured for 6 days in DC culture medium supplemented with 50 ng of recombinant granulocyte-macrophage colony-stimulating factor (rGM-CSF; R&D Systems, Minneapolis, Minn.) per mL and 1000 U of recombinant interleukin-4 (rIL-4; R&D Systems, Minneapolis, Minn.) per mL. ..

    Article Title: MAP4-regulated dynein-dependent trafficking of BTN3A1 controls the TBK1–IRF3 signaling axis
    Article Snippet: Monocytes were isolated from PBMCs using a magnetic bead-based positive-selection kit (IMag) and an anti-human CD14 antibody (BD Biosciences). .. To generate monocyte-derived macrophages (MDMs), CD14-positive cells were treated with human recombinant interleukin 4 (hrIL-4; R&D Systems) and human recombinant granulocyte–macrophage colony-stimulating factor (hrGM-CSF; R&D Systems) for 5 d. All experiments involving human blood were approved by the Institutional Review Board of Seoul National University (SNUIRB no. E1304-001-023). ..

    Article Title: A Randomized Double-Blind Placebo-Controlled Phase I/II Clinical Trial of a Human Papillomavirus Therapeutic Vaccine, PepCan, for Reducing Head and Neck Cancer Recurrence
    Article Snippet: .. Autologous dendritic cells were established by growing monocytes in the presence of granulocyte monocyte-colony stimulating factor (50 ng/mL, Sanofi-Aventis, Paris, France) and recombinant interleukin-4 (100 U/mL, R&D Systems, Minneapolis, MN) for seven days, and were matured by 48-hour culture in wells containing irradiated mouse L-cells expressing CD40 ligands. .. CD3 T cells were magnetically selected (Pan T Cell Isolation Kit II, Miltenyi Biotec, Auburn, CA) from CD14-depleted PBMCs.

    Article Title: A phase I dose-escalation clinical trial of a peptide-based human papillomavirus therapeutic vaccine with Candida skin test reagent as a novel vaccine adjuvant for treating women with biopsy-proven cervical intraepithelial neoplasia 2/3
    Article Snippet: .. Autologous dendritic cells were established by growing monocytes in the presence of granulocyte monocyte-colony stimulating factor (50 ng/mL, Sanofi-Aventis, #420039) and recombinant interleukin-4 (100 U/mL, R&D Systems, # 204-IL-050) for 7 d, and were matured by 48-h culture in wells containing irradiated mouse L-cells expressing CD40 ligands. .. CD3 T cells were magnetically selected (Pan T Cell Isolation Kit II, Miltenyi Biotec, #130-096-535) from CD14-depleted PBMCs.

    Article Title: Human macrophages and monocyte-derived dendritic cells stimulate the proliferation of endothelial cells through midkine production
    Article Snippet: Macrophages were obtained from monocytes cultured for 6 days in RPMI media (Gibco, UK) supplemented with 50 ng/ml human recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D, UK). .. MDDCs were differentiated from monocytes for 6 days in RPMI media supplemented with 50 ng/ml GM-CSF and 50 ng/ml of human recombinant interleukin-4 (IL-4; R&D, UK). .. Cells (10 6 cells/ml) were stimulated for 24 hrs with 20ng/ml lipopolysaccharide (LPS; InvivoGen, USA).

    Irradiation:

    Article Title: A phase I dose-escalation clinical trial of a peptide-based human papillomavirus therapeutic vaccine with Candida skin test reagent as a novel vaccine adjuvant for treating women with biopsy-proven cervical intraepithelial neoplasia 2/3
    Article Snippet: .. Autologous dendritic cells were established by growing monocytes in the presence of granulocyte monocyte-colony stimulating factor (50 ng/mL, Sanofi-Aventis, #420039) and recombinant interleukin-4 (100 U/mL, R&D Systems, # 204-IL-050) for 7 d, and were matured by 48-h culture in wells containing irradiated mouse L-cells expressing CD40 ligands. .. CD3 T cells were magnetically selected (Pan T Cell Isolation Kit II, Miltenyi Biotec, #130-096-535) from CD14-depleted PBMCs.

    Article Title: A Randomized Double-Blind Placebo-Controlled Phase I/II Clinical Trial of a Human Papillomavirus Therapeutic Vaccine, PepCan, for Reducing Head and Neck Cancer Recurrence
    Article Snippet: .. Autologous dendritic cells were established by growing monocytes in the presence of granulocyte monocyte-colony stimulating factor (50 ng/mL, Sanofi-Aventis, Paris, France) and recombinant interleukin-4 (100 U/mL, R&D Systems, Minneapolis, MN) for seven days, and were matured by 48-hour culture in wells containing irradiated mouse L-cells expressing CD40 ligands. .. CD3 T cells were magnetically selected (Pan T Cell Isolation Kit II, Miltenyi Biotec, Auburn, CA) from CD14-depleted PBMCs.

    Article Title: A phase I dose-escalation clinical trial of a peptide-based human papillomavirus therapeutic vaccine with Candida skin test reagent as a novel vaccine adjuvant for treating women with biopsy-proven cervical intraepithelial neoplasia 2/3
    Article Snippet: .. Autologous dendritic cells were established by growing monocytes in the presence of granulocyte monocyte-colony stimulating factor (50 ng/mL, Sanofi-Aventis, #420039) and recombinant interleukin-4 (100 U/mL, R&D Systems, # 204-IL-050) for 7 d, and were matured by 48-h culture in wells containing irradiated mouse L-cells expressing CD40 ligands. .. CD3 T cells were magnetically selected (Pan T Cell Isolation Kit II, Miltenyi Biotec, #130-096-535) from CD14-depleted PBMCs.

    Expressing:

    Article Title: A phase I dose-escalation clinical trial of a peptide-based human papillomavirus therapeutic vaccine with Candida skin test reagent as a novel vaccine adjuvant for treating women with biopsy-proven cervical intraepithelial neoplasia 2/3
    Article Snippet: .. Autologous dendritic cells were established by growing monocytes in the presence of granulocyte monocyte-colony stimulating factor (50 ng/mL, Sanofi-Aventis, #420039) and recombinant interleukin-4 (100 U/mL, R&D Systems, # 204-IL-050) for 7 d, and were matured by 48-h culture in wells containing irradiated mouse L-cells expressing CD40 ligands. .. CD3 T cells were magnetically selected (Pan T Cell Isolation Kit II, Miltenyi Biotec, #130-096-535) from CD14-depleted PBMCs.

    Article Title: A Randomized Double-Blind Placebo-Controlled Phase I/II Clinical Trial of a Human Papillomavirus Therapeutic Vaccine, PepCan, for Reducing Head and Neck Cancer Recurrence
    Article Snippet: .. Autologous dendritic cells were established by growing monocytes in the presence of granulocyte monocyte-colony stimulating factor (50 ng/mL, Sanofi-Aventis, Paris, France) and recombinant interleukin-4 (100 U/mL, R&D Systems, Minneapolis, MN) for seven days, and were matured by 48-hour culture in wells containing irradiated mouse L-cells expressing CD40 ligands. .. CD3 T cells were magnetically selected (Pan T Cell Isolation Kit II, Miltenyi Biotec, Auburn, CA) from CD14-depleted PBMCs.

    Article Title: A phase I dose-escalation clinical trial of a peptide-based human papillomavirus therapeutic vaccine with Candida skin test reagent as a novel vaccine adjuvant for treating women with biopsy-proven cervical intraepithelial neoplasia 2/3
    Article Snippet: .. Autologous dendritic cells were established by growing monocytes in the presence of granulocyte monocyte-colony stimulating factor (50 ng/mL, Sanofi-Aventis, #420039) and recombinant interleukin-4 (100 U/mL, R&D Systems, # 204-IL-050) for 7 d, and were matured by 48-h culture in wells containing irradiated mouse L-cells expressing CD40 ligands. .. CD3 T cells were magnetically selected (Pan T Cell Isolation Kit II, Miltenyi Biotec, #130-096-535) from CD14-depleted PBMCs.

    Isolation:

    Article Title: Functional characterization of biodegradable nanoparticles as antigen delivery system
    Article Snippet: .. Briefly, isolated PBMCs were allowed to adhere to plastic in 6-well plates for 2 h. Adherent monocytes were washed with RPMI 1640 medium and were then cultured for 6 days in DC culture medium supplemented with 50 ng of recombinant granulocyte-macrophage colony-stimulating factor (rGM-CSF; R&D Systems, Minneapolis, Minn.) per mL and 1000 U of recombinant interleukin-4 (rIL-4; R&D Systems, Minneapolis, Minn.) per mL. ..



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